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Literature summary for 3.5.4.B9 extracted from
- Crystal structure of DNA cytidine deaminase ABOBEC3G catalytic deamination domain suggests a binding mode of full-length enzyme to single-stranded DNA (2015), J. Biol. Chem., 290, 4010-4021 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure of a A3G-CD2 head-to-tail dimer in which the N terminus of the monomer H (head) interacts with the C terminus of monomer T (tail). A continuous DNA binding groove is observed | Homo sapiens |
| structure of a novel catalytic domain head-to-tail dimer in which the N terminus of the monomer head | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D264A | 5.1% of wild-type activity | Homo sapiens |
| D264A | 5.1% of wild-type activtiy | Homo sapiens |
| D370A | 15.8% of wild-type activity | Homo sapiens |
| D370A | 15.8% of wild-type activtiy | Homo sapiens |
| F268A | 25.2% of wild-type activity | Homo sapiens |
| F268A | 25.2% of wild-type activtiy | Homo sapiens |
| H248G | 158% of wild-type activity | Homo sapiens |
| H250A | 266% of wild-type activity | Homo sapiens |
| H250A | 266% of wild-type activtiy | Homo sapiens |
| H250G | 158% of wild-type activtiy | Homo sapiens |
| P210A | 1.4% of wild-type activity | Homo sapiens |
| P210A | 1.4% of wild-type activtiy | Homo sapiens |
| P210G | 9.9% of wild-type activity | Homo sapiens |
| P210G | 9.9% of wild-type activtiy | Homo sapiens |
| Q245A | 5.5% of wild-type activity | Homo sapiens |
| Q245A | 5.5% of wild-type activtiy | Homo sapiens |
| Q380A | 68.5% of wild-type activity | Homo sapiens |
| Q380A | 68.5% of wild-type activtiy | Homo sapiens |
| R256A | 1.3% of wild-type activity | Homo sapiens |
| R256A | 1.3% of wild-type activtiy | Homo sapiens |
| R374A | 3.4% of wild-type activity | Homo sapiens |
| R374A | 3.4% of wild-type activtiy | Homo sapiens |
| R376A | 14.9% of wild-type activity | Homo sapiens |
| R376A | 14.9% of wild-type activtiy | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00819 | - |
5'-ATTCCCAATT-3' | mutant P210A, pH 7.5, 20°C | Homo sapiens |  |
| 0.0119 | - |
5'-ATTCCCAATT-3' | mutant D370A, pH 7.5, 20°C | Homo sapiens | |
| 0.0119 | - |
cytidine in HIV-1 virus ssDNA | mutant D370A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0162 | - |
5'-ATTCCCAATT-3' | mutant Q380A, pH 7.5, 20°C | Homo sapiens | |
| 0.0162 | - |
cytidine in HIV-1 virus ssDNA | mutant Q380A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0185 | - |
5'-ATTCCCAATT-3' | mutant P210G, pH 7.5, 20°C | Homo sapiens | |
| 0.0185 | - |
cytidine in HIV-1 virus ssDNA | mutant P210G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0204 | - |
cytidine in HIV-1 virus ssDNA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0204 | - |
5'-ATTCCCAATT-3' | wild-type, pH 7.5, 20°C | Homo sapiens | |
| 0.0225 | - |
5'-ATTCCCAATT-3' | mutant F268A, pH 7.5, 20°C | Homo sapiens | |
| 0.0225 | - |
cytidine in HIV-1 virus ssDNA | mutant F268A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0271 | - |
cytidine in HIV-1 virus ssDNA | mutant R374A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0331 | - |
5'-ATTCCCAATT-3' | mutant H248G, pH 7.5, 20°C | Homo sapiens | |
| 0.0331 | - |
cytidine in HIV-1 virus ssDNA | mutant H248G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0333 | - |
5'-ATTCCCAATT-3' | mutant H250A, pH 7.5, 20°C | Homo sapiens | |
| 0.0333 | - |
cytidine in HIV-1 virus ssDNA | mutant H250A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0632 | - |
5'-ATTCCCAATT-3' | mutant R376A, pH 7.5, 20°C | Homo sapiens | |
| 0.0632 | - |
cytidine in HIV-1 virus ssDNA | mutant R376A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0819 | - |
cytidine in HIV-1 virus ssDNA | mutant P210A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.1639 | - |
5'-ATTCCCAATT-3' | mutant R256A, pH 7.5, 20°C | Homo sapiens | |
| 0.164 | - |
cytidine in HIV-1 virus ssDNA | mutant R256A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.271 | - |
5'-ATTCCCAATT-3' | mutant R374A, pH 7.5, 20°C | Homo sapiens | |
| 0.2785 | - |
5'-ATTCCCAATT-3' | mutant Q245A, pH 7.5, 20°C | Homo sapiens | |
| 0.279 | - |
cytidine in HIV-1 virus ssDNA | mutant Q245A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.3944 | - |
5'-ATTCCCAATT-3' | mutant D264A, pH 7.5, 20°C | Homo sapiens | |
| 0.395 | - |
cytidine in HIV-1 virus ssDNA | mutant D264A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9HC16 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-ATTCCCAATT-3' + H2O | - |
Homo sapiens | 5'-ATTCCUAATT-3' | - |
? | |
| cytidine in HIV-1 virus ssDNA + H2O | - |
Homo sapiens | uridine in HIV-1 virus ssDNA + NH3 | - |
? | |
| additional information | intrinsic DNA cytidine deaminase activity of full-length A3G is measured by expressing these proteins in ung-deficient Escherichia coli BW310 and by quantifying the frequency of RifR-conferring rpoB mutations | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABOBEC3G | - |
Homo sapiens |
| APOBEC3G | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
5'-ATTCCCAATT-3' | mutant P210A, pH 7.5, 20°C | Homo sapiens | |
| 0.0001 | - |
cytidine in HIV-1 virus ssDNA | mutant P210A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0001 | - |
5'-ATTCCCAATT-3' | mutant R374A, pH 7.5, 20°C | Homo sapiens | |
| 0.0001 | - |
cytidine in HIV-1 virus ssDNA | mutant R374A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0002 | - |
5'-ATTCCCAATT-3' | mutant D370A, pH 7.5, 20°C | Homo sapiens | |
| 0.0002 | - |
cytidine in HIV-1 virus ssDNA | mutant D370A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0002 | - |
5'-ATTCCCAATT-3' | mutant P210G, pH 7.5, 20°C | Homo sapiens | |
| 0.0002 | - |
cytidine in HIV-1 virus ssDNA | mutant P210G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0002 | - |
5'-ATTCCCAATT-3' | mutant R256A, pH 7.5, 20°C | Homo sapiens | |
| 0.0005 | - |
5'-ATTCCCAATT-3' | mutant F268A, pH 7.5, 20°C | Homo sapiens | |
| 0.0005 | - |
cytidine in HIV-1 virus ssDNA | mutant F268A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0009 | - |
5'-ATTCCCAATT-3' | mutant R376A, pH 7.5, 20°C | Homo sapiens | |
| 0.0009 | - |
cytidine in HIV-1 virus ssDNA | mutant R376A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.001 | - |
5'-ATTCCCAATT-3' | mutant Q380A, pH 7.5, 20°C | Homo sapiens | |
| 0.001 | - |
cytidine in HIV-1 virus ssDNA | mutant Q380A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0014 | - |
cytidine in HIV-1 virus ssDNA | mutant Q245A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0018 | - |
cytidine in HIV-1 virus ssDNA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0018 | - |
5'-ATTCCCAATT-3' | wild-type, pH 7.5, 20°C | Homo sapiens | |
| 0.0018 | - |
5'-ATTCCCAATT-3' | mutant D264A, pH 7.5, 20°C | Homo sapiens | |
| 0.0018 | - |
cytidine in HIV-1 virus ssDNA | mutant D264A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.002 | - |
cytidine in HIV-1 virus ssDNA | mutant R256A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0047 | - |
5'-ATTCCCAATT-3' | mutant H248G, pH 7.5, 20°C | Homo sapiens | |
| 0.0047 | - |
cytidine in HIV-1 virus ssDNA | mutant H248G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.008 | - |
5'-ATTCCCAATT-3' | mutant H250A, pH 7.5, 20°C | Homo sapiens | |
| 0.008 | - |
cytidine in HIV-1 virus ssDNA | mutant H250A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.014 | - |
5'-ATTCCCAATT-3' | mutant Q245A, pH 7.5, 20°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
5'-ATTCCCAATT-3' | mutant P210A, pH 7.5, 20°C | Homo sapiens | |
| 0.001 | - |
5'-ATTCCCAATT-3' | mutant R256A, pH 7.5, 20°C | Homo sapiens | |
| 0.0012 | - |
cytidine in HIV-1 virus ssDNA | mutant R256A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0013 | - |
cytidine in HIV-1 virus ssDNA | mutant P210A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.003 | - |
5'-ATTCCCAATT-3' | mutant R374A, pH 7.5, 20°C | Homo sapiens | |
| 0.0031 | - |
cytidine in HIV-1 virus ssDNA | mutant R374A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0046 | - |
cytidine in HIV-1 virus ssDNA | mutant D264A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.005 | - |
5'-ATTCCCAATT-3' | mutant D264A, pH 7.5, 20°C | Homo sapiens | |
| 0.005 | - |
5'-ATTCCCAATT-3' | mutant Q245A, pH 7.5, 20°C | Homo sapiens | |
| 0.005 | - |
cytidine in HIV-1 virus ssDNA | mutant Q245A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.009 | - |
5'-ATTCCCAATT-3' | mutant P210G, pH 7.5, 20°C | Homo sapiens | |
| 0.009 | - |
cytidine in HIV-1 virus ssDNA | mutant P210G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0135 | - |
cytidine in HIV-1 virus ssDNA | mutant R376A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.014 | - |
5'-ATTCCCAATT-3' | mutant D370A, pH 7.5, 20°C | Homo sapiens | |
| 0.014 | - |
5'-ATTCCCAATT-3' | mutant R376A, pH 7.5, 20°C | Homo sapiens | |
| 0.0143 | - |
cytidine in HIV-1 virus ssDNA | mutant D370A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0228 | - |
cytidine in HIV-1 virus ssDNA | mutant F268A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.023 | - |
5'-ATTCCCAATT-3' | mutant F268A, pH 7.5, 20°C | Homo sapiens | |
| 0.024 | - |
cytidine in HIV-1 virus ssDNA | mutant H250A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.0618 | - |
cytidine in HIV-1 virus ssDNA | mutant Q380A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.062 | - |
5'-ATTCCCAATT-3' | mutant Q380A, pH 7.5, 20°C | Homo sapiens | |
| 0.09 | - |
cytidine in HIV-1 virus ssDNA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.09 | - |
5'-ATTCCCAATT-3' | wild-type, pH 7.5, 20°C | Homo sapiens | |
| 0.143 | - |
5'-ATTCCCAATT-3' | mutant H248G, pH 7.5, 20°C | Homo sapiens | |
| 0.143 | - |
cytidine in HIV-1 virus ssDNA | mutant H248G, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 0.24 | - |
5'-ATTCCCAATT-3' | mutant H250A, pH 7.5, 20°C | Homo sapiens | |
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